The 2027 24 Hours of Daytona will be the 65th Daytona 24 hours endurance sports car race sanctioned by International Motor Sports Association (IMSA), taking place from 28 to January 31, 2027, at the Daytona International Speedway combined road course in Daytona Beach, Florida. The race will be the opening round of the 2026 IMSA SportsCar Championship season.

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